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| Registros recuperados: 57 | |
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| Martinati,Juliana Camargo; Pacheco,Flávia Tereza Hansen; Miranda,Vitor Fernandes Oliveira de; Tsai,Siu Mui. |
| Strains of Xylella fastidiosa from several hosts (coffee, citrus, grape, almond, oleander, peach, plum, etc.) were characterized by analyzing the content of the nucleotide sequences of 16S-23S rDNA (coding for a small subunit ribosomal RNA) spacer region (ITS). Current methods for sequencing the ITS region yields partial sequences that do not contribute with significant information. According to this fact, new primers have been designed in order to obtain a complete sequence and facilitate the sequencing. The complete 16S-23S sequences from 08 strains were amplified through PCR using the primers designed in our lab. The 16S-23S sequences obtained were compared with 52 others sequences entries in GenBank database. The results revealed a higher level of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; 16S-23S rDNA; Intergenic spacer; Identification; Detection. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100033 |
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| Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Berthe, Franck. |
| Bonamiosis was first reported in association with mass mortality of flat oysters, Ostrea edulis, in June 1979 in L'Ile Tudy, Brittany. The disease rapidly spread to all of the oyster farming areas in France but also in other European countries. The French flat oyster production, which already suffered from another protozoan disease, marteiliosis, decreased from 5500 mt in 1979 to less than 2000 mt after 1980. In 2001, about 350 French farms sell 1650 mt of flat oysters. This production mainly relies on natural spat collection which specially occurs in Quiberon bay. One third of this spat is transferred from South to North Brittany for further growth. Quiberon bay constitutes an interesting site regarding the surveillance of bonamiosis because of the... |
| Tipo: Text |
Palavras-chave: Detection; Bonamia ostreae; Protozoan; Diseases; Pathology; Ostrea edulis; Oysters. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3314.pdf |
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| Luten, J.; Bouquet, W.; Oehlenschläger, J.; Meetschen, U.; Etienne, M.; Stroud, G.; Bykowski, P.; Batista, I.; Vyncke, W.; Stefansson, G.. |
| Eight WEFTA laboratories participated in two series of intercomparison exercises on sulfite determination in tropical shrimps. Samples of tropical shrimps were spiked with sodium metabisulfite (Na<sub>2</sub>S<sub>2</sub>O<sub>5</sub>) and hydroxymethylsulfonate (HMS) at a level of 25-90 mg SO<sub>2</sub>/kg. Most of the laboratories determined the sulfite content with (modified) methods of Monier-Williams or De Vries et al. The overall mean recovery of sulfite was rather low (47-60%), which may be attributed to an irreversible reaction of sulfite with the tropical shrimps. The repeatability of the methods within the participating laboratories was good. However, reproducibility among the laboratories was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Detection; Fishery products; Food technology; International cooperation; Quality control; Recovery; Shrimp fisheries; Sulphites. |
| Ano: 1997 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=5742 |
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| Bosch, Albert; Sanchez, Gloria; Abbaszadegan, Morteza; Carducci, Annalaura; Guix, Susana; Le Guyader, Soizick; Netshikweta, Rembuluwani; Pinto, Rosa M.; Van Der Poel, Wim H. M.; Rutjes, Saskia; Sano, Daisuke; Taylor, Maureen B.; Van Zyl, Walda B.; Rodriguez-lazaro, David; Kovac, Katarina; Sellwood, Jane. |
| Potential ways to address the issues that relate to the techniques for analyzing food and environmental samples for the presence of enteric viruses are discussed. It is not the authors' remit to produce or recommend standard or reference methods but to address specific issues in the analytical procedures. Foods of primary importance are bivalve molluscs, particularly, oysters, clams, and mussels; salad crops such as lettuce, green onions and other greens; and soft fruits such as raspberries and strawberries. All types of water, not only drinking water but also recreational water (fresh, marine, and swimming pool), river water (irrigation water), raw and treated sewage are potential vehicles for virus transmission. Well over 100 different enteric viruses... |
| Tipo: Text |
Palavras-chave: Enteric viruses; Gastroenteritis; Hepatitis; Detection; Concentration. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00031/14191/11465.pdf |
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| Rigouin, Coraline; Delbarre Ladrat, Christine; Sinquin, Corinne; Colliec-jouault, Sylvia; Dion, M. |
| To find biocatalyst allowing depolymerization of new polysaccharides, one needs to get a sensitive and well adapted method to a screening program. This led us to compare biochemical methods of detection of the depolymerization. Currently used methods such as reducing sugars assays, double bond monitoring or molecular weight determination were tested to follow the kinetic of depolymerization with different enzyme / polysaccharide couples. The range of concentrations of assorted enzymes allowed us to identify the most sensitive and appropriate method to detect polysaccharide degradation. Reducing sugars assays are quantitative, sensitive and almost usable with all kind of polysaccharide but some compounds may interfere with them. When polysaccharide is... |
| Tipo: Text |
Palavras-chave: Detection; Enzyme; Depolymerization; Carbohydrate. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4723.pdf |
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| Menezes,Caroline Anunciação; Gonçalves,Danielle Silva; Amianti,Jackeline; Fernandes,Irene; Taddei,Carla Romano; Koga,Paula Célia Mariko; Trabulsi,Luiz Rachid; Martinez,Marina Baquerizo; Piazza,Roxane Maria Fontes. |
| A capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has beendeveloped. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% for sensitivity, 94% for specificity and 92% for efficiency. Thus, the capture immunoassayis a sensitive tool for detection of E. coli, which produces heat-labile enterotoxin, and is suitable for use in clinical laboratories and epidemiological surveys in developing world. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Detection; Polyclonal antibody; Monoclonal antibody; Thermo-labile toxin; E. coli. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500004 |
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| Torre,D De la; Nuñez,LF; Parra,SHS; Astolfi-Ferreira,CS; Ferreira,AJP. |
| ABSTRACT This study determined the presence of Tremovirus A as the possible agent related to Avian Encephalomyelitis in broiler chicks from the states of São Paulo (SP) Paraná (PR), Goiás (GO), Santa Catarina (SC) and Rio Grande do Sul (RS), between the years 2006 and 2015. Samples of the nervous, digestive, respiratory, immune, and renal systems, plus muscular organs from broiler chicks with neurological problems such as ataxia and muscle tremors, and four (4) commercial vaccines as positive control, were tested by reverse-transcriptase (RT-PCR) amplification and DNA sequencing. A highly conserved region (P1) of the viral genome, was used to amplify a segment which encodes a structural protein VP4. Out of 112 samples, 46 were positive (42%) for Tremovirus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken; Tremovirus A; Avian Encephalomyelitis; Detection; Molecular characterization. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300527 |
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| Daste, S. |
| The "CRABE" method is a crack detection technique based on the use of low electromagnetic frequencies. This (patented) method relies on a global injection of current within the structure under inspection. The presence of a crack shall cause a disruption in the current flow, and consequently of the magnetic field off the surface in the vicinity of a defect. It is this magnetic signature which is detected by the magnetic sensor used with "CRABE". The major advantage of this process, developed by TRAVOCEAN and the LETI Division of CEA with the help of IFREMER, appears clearly: No preliminary cleaning of the welds to be inspected is required, since no contact is necessary. Similarly, it shall be possible to inspect the whole of structures to a total cost all... |
| Tipo: Text |
Palavras-chave: Offshore structures; Fouling; Underwater exploration; Steel structures; Sensors; Cracks; Detection; Unmanned vehicles; Marine technology. |
| Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/1990/acte-1161.pdf |
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| Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan. |
| Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1... |
| Tipo: Text |
Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf |
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| Vigneron, Vassilia; Solliec, Gaelle; Montanie, Hélène; Renault, Tristan. |
| Since 1991, herpesvirus infections have been reported among larvae and juveniles of various bivalves. Most of the studies focused on detection of viral infections of economically important species. However, the persistence of bivalve herpesviruses; in the marine environment is poorly documented. The present study concerns the role of seawater parameters in Ostreid Herpesvirus I (OsHV-1) detection by polymerase chain reaction (PCR). Viral DNA extracted from purified particles or virions present in infected oyster larvae were detected by PCR after storage in different media at different temperatures. The lowest detection threshold was found using distilled water or Tris EDTA buffer. In seawater, the threshold was higher. The use of sterile media permitted... |
| Tipo: Text |
Palavras-chave: Temperature; PCR; Detection; Seawater; Viral DNA; Oyster herpesvirus 1. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-2909.pdf |
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| Castagna,Sandra Maria Ferraz; Muller,Monika; Macagnan,Marisa; Rodenbusch,Carla Rosane; Canal,Cláudio Wageck; Cardoso,Marisa. |
| The aim of this study was to compare a polymerase chain reaction (PCR) method combined with selective enrichment in Rappaport-Vassiliadis broth (PCR-RVB) with standard microbiological techniques (SMT) for the generic detection of Salmonella in samples of porcine origin. Two hundred sixty eight field samples consisting of 42 sets of pooled porcine mandibular lymph nodes and tonsils, 44 samples of intestinal content, 38 pork sausage meat samples and 144 samples of feed collected from swine farms were submitted to the PCR-RVB and SMT protocols. Salmonella was detected in 54 samples using the PCR-RVB assay and in 42 samples by SMT, three of the SMT Salmonella-positive samples (one each of S. Derby, S. Panama and S. Typhimurium) being Salmonella-negative by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Detection; PCR; Pork; Salmonella; Swine. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400013 |
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| Varsamos, S.; Pepin, Jean-francois; Sauvage, Christopher; Renault, Tristan. |
| OsHV-1 was the first herpesvirus to be identified in an invertebrate host and is associated to mortalities in the Pacific oyster (Crassostrea gigas) and other economically important bivalve species. Infections due to OsHV-1 are frequently reported and cause high mortality rates in larvae and spat of C. gigas. Detection of this pathogen is usually performed by PCR and/or in situ hybridization. This presentation will focus on the development of a new molecular diagnostic tool for OsHV-1 (and OsHV-1 variant 1) detection: the mini-array. This detection system, which allows multiple pathogens detection, combines viral DNA amplification by PCR and a specific hybridisation on a nylon membrane, revealed by a colorimetric reaction (DNA chip). Multiplex PCR involves... |
| Tipo: Text |
Palavras-chave: Diagnostic; Crassotrea gigas; Oyster; Detection; Herpesviridae; OsHV 1. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3652.pdf |
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| Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter. |
| Little information is available on viral infections that affect bivalve molluscs. Such a lack of data is due ta a certain inadequacy of the diagnosis methods that are employed when massive mortality events occur. Most laboratories involved in mollusc pathology still analyse samples through light microscopy. World-wide, there is thus currently a lack of information concerning the occurrence of bivalve herpesvituses. This is probably due to the lack of suitable diagnostic tools. The basic method for identification and examination of suspect samples is predominantly histopathology. This enables the identification of any cellular changes, but is not conclusive identification of bivalve herpesviruses. This technique doesn't allow, by itself, to detect viruses... |
| Tipo: Text |
Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00044/15525/12912.pdf |
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| Registros recuperados: 57 | |
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